6P4P
Salmonella typhi PltB Homopentamer N29K Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | RIGAKU HyPic-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.509, 96.350, 118.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.253 - 2.000 |
| R-factor | 0.1844 |
| Rwork | 0.183 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.855 |
| Data reduction software | CrysalisPro (40.43a) |
| Data scaling software | CrysalisPro (40.43a) |
| Phasing software | PHENIX (1.12-2829) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.256 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 47275 | 4734 |
| <I/σ(I)> | 30.55 | |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG1500, Sodium actetate pH 4.4 |
