6P4N
Salmonella typhi PltB Homopentamer with Neu5Ac-alpha-2-6-Gal-beta-1-4-GlcNAc Glycans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.87 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.736, 98.483, 105.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.694 - 1.700 |
| R-factor | 0.1771 |
| Rwork | 0.177 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | HKL-2000 (717) |
| Data scaling software | HKL-2000 (717) |
| Phasing software | PHENIX (1.10.1-2155) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 79595 | 3755 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG1500, Sodium actetate pH 4.4 |
