6P3X

Crystal Structure of Full Length APOBEC3G E/Q (pH 7.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	67.495, 153.241, 67.494
Unit cell angles	90.00, 114.87, 90.00

Refinement procedure

Resolution	47.835 - 2.402
R-factor	0.1906
Rwork	0.189
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	47476	4210
<I/σ(I)>	18.3
Completeness [%]	98.3	87.7
Redundancy	6.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	50 mM HEPES, pH 7 1.6 M ammonium sulfate 10 mM MgCl2