6P22

Photorhabdus Virulence Cassette (PVC) PAAR repeat protein Pvc10 in complex with a T4 gp5 beta-helix fragment modified to mimic Pvc8, the central spike protein of PVC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	34.305, 56.963, 222.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.213 - 2.291
R-factor	0.184
Rwork	0.182
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jj2
RMSD bond length	0.002
RMSD bond angle	0.483
Data reduction software	XDS (March 30, 2013)
Data scaling software	X-GEN (March 30, 2013)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.290	2.290
Rmeas	0.125	0.702
Number of reflections	37669	5838
<I/σ(I)>	9.78	2.05
Completeness [%]	99.2	96
Redundancy	6.2
CC(1/2)	0.998	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	MPD 28-34% PEG 2000 8-18% 100 mM Imidazole pH 8.0