6P20

Bacteriophage phiKZ gp163.1 PAAR repeat protein in complex with a T4 gp5 beta-helix fragment modified to mimic the phiKZ central spike gp164

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	34.427, 66.597, 173.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.384 - 1.749
R-factor	0.1492
Rwork	0.148
R-free	0.19140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jj2
RMSD bond length	0.003
RMSD bond angle	0.643
Data reduction software	XDS (March 30, 2013)
Data scaling software	XDS (March 30, 2013)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.749	1.750
Rmeas	0.055	0.552
Number of reflections	77426	12230
<I/σ(I)>	18.17	2.63
Completeness [%]	99.3	97.3
Redundancy	3.4	3.2
CC(1/2)	1.000	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20-30% PEG 3350 100 mM MES ph 6.5 200-350 mM Mg(NO3)2