6P1Z

Bacteriophage phiKZ gp163.1 PAAR repeat protein in complex with the C-terminal part of the T4 gp5 beta-helical domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	72.275, 69.123, 77.802
Unit cell angles	90.00, 102.48, 90.00

Refinement procedure

Resolution	44.597 - 2.099
R-factor	0.19
Rwork	0.188
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jj2
RMSD bond length	0.002
RMSD bond angle	0.441
Data reduction software	XDS (March 30, 2013)
Data scaling software	XDS (March 30, 2013)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.099	2.100
Rmeas	0.120	0.483
Number of reflections	82024	12480
<I/σ(I)>	6.93	2
Completeness [%]	95.4	89.3
Redundancy	1.8	1.72
CC(1/2)	0.998	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	293	47.5-50 % PEG5500 MME 100 mM Glycine pH 10 25-100 mM KBr