6P1Z
Bacteriophage phiKZ gp163.1 PAAR repeat protein in complex with the C-terminal part of the T4 gp5 beta-helical domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.275, 69.123, 77.802 |
| Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
| Resolution | 44.597 - 2.099 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jj2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.441 |
| Data reduction software | XDS (March 30, 2013) |
| Data scaling software | XDS (March 30, 2013) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.099 | 2.100 |
| Rmeas | 0.120 | 0.483 |
| Number of reflections | 82024 | 12480 |
| <I/σ(I)> | 6.93 | 2 |
| Completeness [%] | 95.4 | 89.3 |
| Redundancy | 1.8 | 1.72 |
| CC(1/2) | 0.998 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 47.5-50 % PEG5500 MME 100 mM Glycine pH 10 25-100 mM KBr |
