6P0X

Structure of the N-terminal domain of effector protein SpvB from Salmonella typhimurium strain LT2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 43 21 2
Unit cell lengths	101.351, 101.351, 211.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 2.400
R-factor	0.1887
Rwork	0.187
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4igl
RMSD bond length	0.003
RMSD bond angle	0.622
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rpim	0.019	0.222
Number of reflections	44119	2160
<I/σ(I)>	41.29
Completeness [%]	100.0	99.8
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	0.1 M Bis-Tris pH 6.5, 14% poly(acrylic acid) 5100 sodium salt, 4% PEG3350, 1 mM DTT and 20 mM MgCl2