6P0U

Crystal structure of ternary DNA complex " FX(1-2)-2Xis" containing E. coli Fis and phage lambda Xis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	151.850, 151.850, 120.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.920 - 3.300
R-factor	0.194
Rwork	0.192
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iv5
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.6.1)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.920	75.920	3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge	0.107	0.023	0.819
Rmeas	0.114	0.026	0.872
Total number of observations	169400
Number of reflections	20814	273	1491
<I/σ(I)>	15.14	64.45	2.24
Completeness [%]	96.0	91.6	96.1
Redundancy	8.139	7.344	8.314
CC(1/2)	0.999	0.999	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	15% PEG 4000