6P0Q

Crystal Structure of Ubiquitin-like Domain of Human WDR12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-04
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	41.197, 50.910, 47.811
Unit cell angles	90.00, 94.11, 90.00

Refinement procedure

Resolution	23.860 - 1.720
R-factor	0.1767
Rwork	0.174
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtc
RMSD bond length	0.007
RMSD bond angle	1.358
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.750
High resolution limit [Å]	1.720	4.670	1.720
Rmerge	0.076	0.040	0.438
Rmeas	0.084	0.044	0.518
Rpim	0.034	0.018	0.269
Total number of observations	117773
Number of reflections	20767	1082	808
<I/σ(I)>	10.7
Completeness [%]	98.6	99.2	78.4
Redundancy	5.7	5.7	3
CC(1/2)		0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	2.5M NH4SO4, 0.1M NaOAc pH4.6