6P0G

N-terminal domain of Thermococcus Gammatolerans McrB bound to m5C DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	41.166, 57.304, 107.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.754 - 2.270
R-factor	0.2405
Rwork	0.238
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.754	2.450
High resolution limit [Å]	2.270	2.270
Number of reflections	17710	2477
<I/σ(I)>	18	2.59
Completeness [%]	99.4	100
Redundancy	4.11	3.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M HEPES pH 7.5, 18% PEG 3350, 0.20 M Ammonium sulfate