6P0G
N-terminal domain of Thermococcus Gammatolerans McrB bound to m5C DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.166, 57.304, 107.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.754 - 2.270 |
| R-factor | 0.2405 |
| Rwork | 0.238 |
| R-free | 0.28930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.754 | 2.450 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 17710 | 2477 |
| <I/σ(I)> | 18 | 2.59 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.11 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES pH 7.5, 18% PEG 3350, 0.20 M Ammonium sulfate |
