6OZX

Wild type GapR crystal structure 1 from C. crescentus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	I 41 3 2
Unit cell lengths	116.481, 116.481, 116.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.553 - 1.851
R-factor	0.194
Rwork	0.193
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.209
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.360	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.113	2.468
Rmeas	0.114	2.491
Rpim	0.014	0.332
Total number of observations	821532
Number of reflections	11872	709
<I/σ(I)>	30.2
Completeness [%]	99.9	98.2
Redundancy	69.2	54.7
CC(1/2)	0.999	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	295	0.16 M ammonium sulfate 12 % PEG 3350 1 % 1,2-butanediol