6OZJ

Crystal structure of Mus musculus (Mm) Endonuclease V in complex with a 23mer RNA oligo containing an inosine in the absence of divalent cation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	72.341, 73.582, 154.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.873 - 2.247
R-factor	0.1583
Rwork	0.156
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.15)
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.920	48.920	2.320
High resolution limit [Å]	2.250	8.990	2.250
Total number of observations	40002	715	3554
Number of reflections	40002	715	3554
<I/σ(I)>	15	35.9	4.1
Completeness [%]	99.8	99.3	98.3
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M potassium sodium tartrate, 20-25% w/v PEG3350