6OZ9
Ebola virus glycoprotein in complex with EBOV-520 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 217.970, 217.970, 217.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.064 - 3.462 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.589 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15rc1_3420) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.064 | 3.586 |
| High resolution limit [Å] | 3.462 | 3.462 |
| Number of reflections | 23638 | 2296 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 36.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES pH 7.0 and 1.4 M Ammonium sulfate |
