6OXM
Crystal structure of L,L-diaminopimelate aminotransferase (DapL) from Verrucomicrobium spinosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2018-07-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95366 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.050, 121.372, 76.545 |
| Unit cell angles | 90.00, 102.01, 90.00 |
Refinement procedure
| Resolution | 47.190 - 2.250 |
| R-factor | 0.19088 |
| Rwork | 0.189 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qgu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.281 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0241) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.460 | 49.460 | 2.380 |
| High resolution limit [Å] | 2.250 | 8.590 | 2.300 |
| Rmerge | 0.145 | 0.044 | 1.334 |
| Rmeas | 0.172 | 0.051 | 1.583 |
| Rpim | 0.092 | 0.027 | 0.843 |
| Total number of observations | 3180 | 15896 | |
| Number of reflections | 44544 | 890 | 4479 |
| <I/σ(I)> | 8.1 | 20.3 | 1.9 |
| Completeness [%] | 99.5 | 99.5 | 95.7 |
| Redundancy | 3.5 | 3.6 | 3.5 |
| CC(1/2) | 0.926 | 0.996 | 0.335 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281.15 | 60% v/v T-mate, 100 mM malic acid |
