6OXB

First bromo-adjacent homology (BAH) domain of human Polybromo-1 (PBRM1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97358
Spacegroup name	P 1
Unit cell lengths	52.304, 52.255, 120.100
Unit cell angles	89.11, 88.24, 60.12

Refinement procedure

Resolution	30.402 - 1.860
R-factor	0.1795
Rwork	0.179
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w4s
RMSD bond length	0.007
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.12-2829_final)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.402	1.910
High resolution limit [Å]	1.860	1.860
Rmerge	0.047	0.269
Rmeas	0.066	0.377
Rpim	0.047	0.267
Number of reflections	83560	3552
<I/σ(I)>	10.1	1.82
Completeness [%]	90.3	50.7
Redundancy	1.67	1.49
CC(1/2)	0.996	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	0.1 M Tris HCl pH 8.0 18% sucrose 1.9 M Ammonium sulfate