6OX6
Crystal structure of the complex between the Type VI effector Tas1 and its immunity protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.631, 66.631, 148.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.170 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.23070 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.083 | 0.322 |
| Rpim | 0.025 | 0.125 |
| Number of reflections | 18200 | 856 |
| <I/σ(I)> | 33.39 | 6.2 |
| Completeness [%] | 98.0 | 96.4 |
| Redundancy | 9.2 | |
| CC(1/2) | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1 M sodium acetate, pH 4.5, 0.8 M sodium phosphate monobasic, 1.2 M potassium phosphate dibasic, cryoprotectant: 20% v/v ethylene glycol |
