6OWU

THE PRP8 INTEIN OF CRYPTOCOCCUS NEOFORMANS in complex with Zn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	ADSC HF-4M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	63.093, 63.039, 78.636
Unit cell angles	94.86, 103.13, 117.44

Refinement procedure

Resolution	40.089 - 1.840
R-factor	0.2
Rwork	0.199
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.893
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.090	1.910
High resolution limit [Å]	1.840	1.840
Rmerge	0.084	0.473
Rmeas	0.106	0.606
Rpim	0.064	0.374
Number of reflections	71327	6013
<I/σ(I)>	9.38	1.6
Completeness [%]	80.4	67.6
Redundancy	2.4	2.2
CC(1/2)	0.994	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	28% PEG4,000, 0.1 M SODIUM ACETATE, PH 4.2, 0.2 M AMMONIUM ACETATE