6OWL

RNA oligonucleotides with 3'-arabino guanosine co-crystallized with GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-29
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	43.483, 43.483, 127.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.000 - 2.000
R-factor	0.24152
Rwork	0.237
R-free	0.31721
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8d
RMSD bond length	0.017
RMSD bond angle	2.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.564
Rmeas	0.090	0.627
Number of reflections	3409	332
<I/σ(I)>	19.3	2.3
Completeness [%]	99.7	100
Redundancy	7.1	5.3
CC(1/2)	0.982	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.08 M Sodium chloride, 0.012 M Potassium chloride, 0.02 M Magnesium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate pH 6.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride