6OW2

X-ray Structure of Polypeptide Deformylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	175
Detector technology	CCD
Collection date	2006-11-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	50.334, 50.334, 91.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.850 - 1.700
R-factor	0.1994
Rwork	0.198
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.796
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	26688	1515
<I/σ(I)>	18
Completeness [%]	88.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1 % - 4 % PEG400 40% - 56% Ammonium Sulfate 0.1 HEPES