6OVM

Crystal Structure of the Pseudomonas capeferrum Anti-sigma Regulator PupR C-terminal Cell-surface Signaling Domain in Complex with the Outer Membrane Transporter PupB N-terminal Signaling Domain (SeMet)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	43.574, 44.714, 141.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.630 - 1.600
R-factor	0.1541
Rwork	0.153
R-free	0.18370
Structure solution method	SAD
RMSD bond angle	1.349
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.340	1.530
High resolution limit [Å]	1.510	1.510
Number of reflections	68598	1511
<I/σ(I)>	18.9
Completeness [%]	98.0
Redundancy	5.9
CC(1/2)	0.999	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	200mM sodium potassium tartrate, 20% PEG 3350