6OVF

Crystal Structure of the Disabled-2 (Dab2) Dab Homology Domain in Complex with Peptide STA03

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.181
Spacegroup name	P 3 2 1
Unit cell lengths	102.644, 102.644, 80.202
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.849 - 1.950
R-factor	0.1786
Rwork	0.175
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7e
RMSD bond length	0.007
RMSD bond angle	0.793
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.850	1.976
High resolution limit [Å]	1.950	1.950
Rmerge		0.642
Rmeas		0.673
Number of reflections	35885	8485
<I/σ(I)>	23.04	4.51
Completeness [%]	100.0	100
Redundancy	10.92
CC(1/2)		0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	40 mM MgCl*6H2O, 5 mM Ni(II)Cl*6H2O, 0.1 M HEPES pH 7.0, 18% (w/v) PEG3350