6OVB
Crystal structure of a Bacillus thuringiensis Cry1Da tryptic core variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 126.177, 126.177, 126.219 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.307 - 2.611 |
| R-factor | 0.1608 |
| Rwork | 0.158 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4arx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.620 | 2.704 |
| High resolution limit [Å] | 2.611 | 2.611 |
| Rmerge | 0.167 | 0.534 |
| Number of reflections | 35721 | 3537 |
| <I/σ(I)> | 19.25 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 0.1 M sodium acetate, pH 4.0, 10% PGME 2000 |
