6OU3

Crystal Structure of the D478S Variant of the Myocilin Olfactomedin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2018-07-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.353, 57.241, 90.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.544 - 1.796
R-factor	0.1517
Rwork	0.150
R-free	0.17310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.811
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.544	1.860
High resolution limit [Å]	1.796	1.796
Rmerge	0.127
Number of reflections	22530	2139
<I/σ(I)>	15.02	2.9
Completeness [%]	99.6
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	20% PEG 3350, 0.2 M Bis Tris pH 6.0, 0.4 M Magnesium formate