6OTX

Crystallographic Structure of (HbII-HbIII)-O2 from Lucina pectinata at pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.19499
Spacegroup name	P 42 21 2
Unit cell lengths	74.736, 74.736, 152.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.558 - 2.539
R-factor	0.1952
Rwork	0.192
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pt8
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.539	2.540
Number of reflections	15012	784334
<I/σ(I)>	36.6
Completeness [%]	99.6
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	7	293	Sodium Formate