6OT6
Rat ERK2 D319N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .97903 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.993, 70.464, 60.697 |
| Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
| Resolution | 29.900 - 1.650 |
| R-factor | 0.1696 |
| Rwork | 0.168 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1erk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.703 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 45537 | 2250 |
| <I/σ(I)> | 18.9 | 1.9 |
| Completeness [%] | 98.0 | 96.4 |
| Redundancy | 7.4 | 6.8 |
| CC(1/2) | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 7.5 mg/ml ERK2 D319N in 25 mM Tris-Cl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 5 mM DTT was mixed with an equal volume of reservoir solution containing 1.5 M ammonium sulfate, 100 mM HEPES pH 7.75, and 2% (v/v) PEG 500 MME |
