6OSX

Crystal structure of uncharacterized protein ECL_02694

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-05
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	61.219, 61.219, 84.534
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.915 - 1.450
R-factor	0.1469
Rwork	0.145
R-free	0.18120
Structure solution method	MIRAS
Starting model (for MR)	SAD data set
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.450	3.940	1.450
Rmerge	0.051	0.038	0.342
Rmeas	0.057	0.043	0.440
Rpim	0.025	0.019	0.273
Total number of observations	114305
Number of reflections	26979	1649	530
<I/σ(I)>	11.6
Completeness [%]	84.4	99.8	33
Redundancy	4.2	5	1.8
CC(1/2)		0.996	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	297	0.8M Lithium sulfate, 0.1M Sodium Acetate, 4% PEG200