6OSI

Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (near cognate)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	210.090, 450.320, 622.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.850 - 4.140
R-factor	0.25
Rwork	0.248
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
RMSD bond length	0.007
RMSD bond angle	1.377
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.845	49.845	4.250
High resolution limit [Å]	4.140	18.510	4.140
Rmerge	0.240	0.056	1.731
Rmeas	0.261	0.060	2.006
Total number of observations	2861036
Number of reflections	439416	4879	31428
<I/σ(I)>	5.07	20.43	0.93
Completeness [%]	98.4	89.8	95.8
Redundancy	6.511	6.277	3.604
CC(1/2)	0.998	0.998	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC