6OSD

Coiled-coil Trimer with Glu:Phe:Lys Triad

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-20
Detector	APEX II CCD
Wavelength(s)	1.5406
Spacegroup name	H 3
Unit cell lengths	38.895, 38.895, 103.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.630 - 2.150
R-factor	0.1848
Rwork	0.177
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2906)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.630	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.193	0.406
Rmeas	0.205	0.463
Rpim	0.068	0.220
Number of reflections	3174	313
<I/σ(I)>	9.4	2.1
Completeness [%]	99.7
Redundancy	7.7
CC(1/2)	0.927	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	40% PEG300, 100 mM sodium cacodylate/HCl, pH 6.5, 200 mM calcium acetate