6OS8
Coiled-coil Trimer with Glu:p-fluorophenylalanine:Lys Triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-04 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 39.066, 39.066, 98.623 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.926 - 2.150 |
| R-factor | 0.2071 |
| Rwork | 0.200 |
| R-free | 0.27830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxt |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2906) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.163 | 0.353 |
| Rmeas | 0.184 | 0.454 |
| Rpim | 0.083 | 0.282 |
| Number of reflections | 3077 | 321 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.1 | |
| CC(1/2) | 0.972 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 40% PEG300, 100 mM sodium cacodylate/HCl, pH 6.5, 200 mM calcium acetate |
