6ORJ
Central spike of phiKZ phage tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 82.595, 82.595, 345.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.889 - 2.370 |
| R-factor | 0.1768 |
| Rwork | 0.175 |
| R-free | 0.20580 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.082 | 0.876 |
| Rmeas | 0.086 | 0.890 |
| Number of reflections | 51342 | 6654 |
| <I/σ(I)> | 22.92 | 1.85 |
| Completeness [%] | 95.1 | 76.1 |
| Redundancy | 10.1 | 5.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 4 M sodium chloride, 100 mM CHES, pH 9.0, 0.3% w/v sorbitol or adonitol |
