6ORG
Crystal structure of SpGH29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.920, 117.160, 64.901 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 41.540 - 1.720 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.614 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.540 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.082 | 0.425 |
| Rpim | 0.031 | 0.162 |
| Number of reflections | 93880 | 13538 |
| <I/σ(I)> | 16.1 | 4.8 |
| Completeness [%] | 98.0 | 96.6 |
| Redundancy | 7.8 | 7.8 |
| CC(1/2) | 0.998 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 16 % PEG 3350, 0.15 M KCl, 1 mM DTT and 0.1 M Bis-Tris. |
