6ORF
Crystal structure of SpGH29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.991, 98.982, 79.090 |
| Unit cell angles | 90.00, 97.57, 90.00 |
Refinement procedure
| Resolution | 49.540 - 1.700 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.433 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.400 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.307 |
| Rpim | 0.035 | 0.165 |
| Number of reflections | 113304 | 16592 |
| <I/σ(I)> | 13.4 | 4.2 |
| Completeness [%] | 96.7 | 97.1 |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 0.997 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 21 % PEG 4000, 0.22 M NaOAc, 1 mM DTT and 0.1 M Tris. |
