6OQZ
Crystal structure of Glucose Isomerase from Non-merohedrally twinned crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER TURBO X-RAY SOURCE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-25 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.931, 97.935, 102.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.880 - 1.600 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.820 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SHELXDE |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.880 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Total number of observations | 125668 | |
| Number of reflections | 61943 | 2184 |
| <I/σ(I)> | 27.01 | 3.93 |
| Completeness [%] | 99.9 | 98.1 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.05 mM TrisHCl buffer pH 7.5, 0.1 M MnCl2 and 14% MPD |
