6OQQ
Legionella pneumophila SidJ/Saccharomyces cerevisiae calmodulin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97927 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.490, 104.749, 107.770 |
Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
Resolution | 46.830 - 2.102 |
R-factor | 0.2026 |
Rwork | 0.202 |
R-free | 0.23360 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.075 | 0.054 | 0.814 |
Rmeas | 0.078 | 0.057 | 0.901 |
Rpim | 0.021 | 0.016 | 0.377 |
Total number of observations | 1496151 | ||
Number of reflections | 130441 | 6774 | 5807 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 98.8 | 99.9 | 88.8 |
Redundancy | 11.5 | 13.4 | 4.8 |
CC(1/2) | 0.998 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M sodium malonate, 0.01 M sodium chloride, 0.001 M magnesium chloride, 0.001 mM calcium chloride, 0.001 M AMP-CPP, 16-17% PEG 3350, 35-40% ethylene glycol |