6OPF

Crystal structure of dmNxf2 UBA domain fused with Panoramix helix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	47.890, 72.890, 59.680
Unit cell angles	90.00, 109.90, 90.00

Refinement procedure

Resolution	42.981 - 2.000
R-factor	0.2207
Rwork	0.220
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oai
RMSD bond length	0.007
RMSD bond angle	0.809
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.030	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.492
Number of reflections	24962	3660
<I/σ(I)>	10.1	2.7
Completeness [%]	95.7	97.2
Redundancy	5.4	5.3
CC(1/2)	0.998	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.095 M Sodium citrate pH 5.6, 19% (v/v) Isopropanol, 19% (w/v) PEG 4000, 5% (v/v) Glycerol