6OOL
Structural elucidation of the Ectodomain of mouse UNC5H2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-16 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.051, 76.890, 159.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.780 - 2.800 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fft |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.971 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 159.010 | 79.505 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.057 | 0.700 | |
| Rmeas | 0.172 | 0.062 | 0.762 |
| Rpim | 0.068 | 0.026 | 0.300 |
| Total number of observations | 111360 | 3567 | 16304 |
| Number of reflections | 17631 | 639 | 2532 |
| <I/σ(I)> | 7.7 | 14.9 | 2.3 |
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 6.3 | 5.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 4.6 mg/ml UNC5HC, 100 mM HEPES, 3M NaCl |
