6OOG

Crystal structure of triosephosphate isomerase from Taenia Solium in complex with 2PG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-08-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	P 41 21 2
Unit cell lengths	66.370, 66.370, 155.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.044 - 2.020
R-factor	0.1863
Rwork	0.184
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3th6
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.044	2.070
High resolution limit [Å]	2.020	2.020
Rmerge	0.298	2.307
Rmeas	0.314	2.433
Rpim	0.095	0.757
Total number of observations		16512
Number of reflections	23571	1693
<I/σ(I)>	7.2	1.2
Completeness [%]	99.7	99.2
Redundancy	10.3	9.8
CC(1/2)	0.992	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.2M Sodium acetate trihydrate 0.1M Sodium cacodylate trihydrate 6.5 30% w/v Polyethylene glycol 8000