6ONU
Complex structure of WhiB1 and region 4 of SigA in P21 space group.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.803, 144.439, 59.890 |
| Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
| Resolution | 36.870 - 1.850 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ono |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.061 | 1.420 |
| Number of reflections | 55679 | 2854 |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 14 | 14.4 |
| CC(1/2) | 0.999 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 100 mM Bis-Tris, pH 5.5, 200 mM magnesium chloride, 25% PEG3350 |
