6ONP

Crystal structure of periplasmic binding protein XoxJ from Methylobacterium extorquens AM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.900, 51.759, 51.241
Unit cell angles	90.00, 98.24, 90.00

Refinement procedure

Resolution	49.390 - 2.270
R-factor	0.2414
Rwork	0.239
R-free	0.27900
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CRANK2
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.270	2.270
Rmerge	0.088	0.674
Rpim	0.037	0.304
Number of reflections	12159	590
<I/σ(I)>	19.6
Completeness [%]	93.7
Redundancy	5.4
CC(1/2)		0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris pH 9.0, 18% PEG 6000