6ON3
A substrate bound structure of L-DOPA dioxygenase from Streptomyces sclerotialus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.830, 40.720, 128.430 |
| Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
| Resolution | 44.010 - 2.310 |
| Rwork | 0.235 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.552 | |
| Number of reflections | 42332 | |
| Completeness [%] | 94.5 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2 and 16% PEG 8000 |
