6OMX
Crystal structure of alr5209, a pentapeptide repeat protein from Nostoc Pcc 7120, determined at 1.7 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 71.001, 27.835, 60.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.501 - 1.710 |
| R-factor | 0.2184 |
| Rwork | 0.215 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS (1.1.7) |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | BALBES (1.1.5) |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.039 | 0.596 |
| Rmeas | 0.042 | 0.647 |
| Rpim | 0.017 | 0.248 |
| Number of reflections | 13612 | 1946 |
| <I/σ(I)> | 22 | |
| Completeness [%] | 98.8 | 99.6 |
| Redundancy | 2 | 6.6 |
| CC(1/2) | 1.000 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.15 M Potassium sodium tartrate tetrahydrate, 0.075 M Sodium citrate tribasic dihydrate pH 5.6, 1.5 M Ammonium sulfate, 25% v/v Glycerol |
