6OMT

HIV-1 capsid hexamer R18D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 6
Unit cell lengths	90.662, 90.662, 56.576
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.900 - 2.052
R-factor	0.1832
Rwork	0.181
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.126	0.134
Number of reflections	16794
<I/σ(I)>	12.2
Completeness [%]	99.7
Redundancy	5.5
CC(1/2)	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293.15	0.1 M Tris-HCl, pH 8.5, 8% PEG8000