6OMI

Crystal structure of the Legionella effector protein MavL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2018-01-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.993054
Spacegroup name	C 1 2 1
Unit cell lengths	97.619, 138.009, 108.523
Unit cell angles	90.00, 92.23, 90.00

Refinement procedure

Resolution	45.140 - 2.643
R-factor	0.2126
Rwork	0.211
R-free	0.25290
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	2.738
High resolution limit [Å]	2.643	2.643
Rmerge	0.078	1.387
Number of reflections	41740	4058
<I/σ(I)>	21.53
Completeness [%]	99.3
Redundancy	1.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	20% PEG8000, 0.2M Ammonium sulfate, 0.1M MES pH 6.5