6OME
Crystal structure of a probable cytosol aminopeptidase (Leucine aminopeptidase, LAP) from Chlamydia trachomatis D/UW-3/Cx
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 137.960, 137.960, 128.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.158 - 1.950 |
R-factor | 0.1444 |
Rwork | 0.143 |
R-free | 0.17140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zi6 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.15_3459)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.158 | 45.158 | 2.000 |
High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
Rmerge | 0.065 | 0.030 | 0.570 |
Rmeas | 0.067 | 0.031 | 0.591 |
Number of reflections | 52788 | 699 | 3844 |
<I/σ(I)> | 27.75 | 63.76 | 5.27 |
Completeness [%] | 100.0 | 98.3 | 100 |
Redundancy | 14.566 | 11.702 | 14.82 |
CC(1/2) | 1.000 | 0.999 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 287 | ChtrB.00799.a.B1.PW38523 at 16.07 mg/mL with 2 mM L-Leucine set up against MCSG3 screen condition C8: 0.1 M Tris pH 8.5, 3.2 M NaCl; supplemented with 20% ethylene glycol as cryo-protectant; unique puck ID ejv5-2, crystal tracking ID 305504c8 |