6OM5
Structure of a haemophore from Haemophilus haemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.45866, 0.95372 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.043, 91.043, 97.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.490 - 1.600 |
| R-factor | 0.17186 |
| Rwork | 0.171 |
| R-free | 0.18449 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.453 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.520 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.069 | 0.790 |
| Rmeas | 0.073 | 0.820 |
| Rpim | 0.023 | 0.250 |
| Number of reflections | 62031 | 3086 |
| <I/σ(I)> | 23.2 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 21 |
| CC(1/2) | 1.000 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1 M sodium acetate trihydrate pH 4.5, 2 M ammonium sulfate, 30 mM sodium phosphate, 72 mM NaCl |
