6OM4
The structure of Microcin C7 biosynthetic enzyme MccB in complex with N-formylated MccA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-01-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.094, 76.059, 131.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.980 - 1.700 |
| R-factor | 0.15692 |
| Rwork | 0.155 |
| R-free | 0.19868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h9g |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.980 | 1.725 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 59837 | 4320 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 96.1 | |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 282 | 0.1 M Magnesium formate, 15% w/v Polyethylene glycol 3350, and 6% w/v 1,6-Hexanediol |
