6OLQ

Protein Tyrosine Phosphatase 1B (1-301), P188A mutant, apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-14
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	88.517, 88.517, 104.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.738 - 2.100
R-factor	0.2047
Rwork	0.203
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qkq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.740	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.075	0.235
Rmeas	0.082	0.257
Rpim	0.031	0.101
Number of reflections	27420	2728
<I/σ(I)>	16.6
Completeness [%]	97.7	99.2
Redundancy	6.9	6.3
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1M Hepes, 0.2 M magnesium acetate and 15-20% polyethylene glycol 8000