6OL4

Protein Tyrosine Phosphatase 1B (1-301), F182A mutant, apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	88.086, 88.086, 103.741
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.490 - 2.150
R-factor	0.2177
Rwork	0.216
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qkq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.800	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.108	0.726
Rmeas	0.113
Number of reflections	25757	2505
<I/σ(I)>	11.35
Completeness [%]	96.2	99.3
Redundancy	9.8
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1M Hepes, 0.2 M magnesium acetate and 15-20% polyethylene glycol 8000