6OL3
Crystal structure of an adenovirus virus-associated RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 100.455, 100.455, 130.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.824 - 2.740 |
| R-factor | 0.2188 |
| Rwork | 0.217 |
| R-free | 0.23530 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.410 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.810 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.152 | 3.642 |
| Rmeas | 3.884 | |
| Number of reflections | 18246 | 1310 |
| <I/σ(I)> | 12.2 | 0.82 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 8.2 |
| CC(1/2) | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM sodium cacodylate (pH 6.5) 28-30% 2-methyl-2,4-pentanediol (MPD) |
