6OKH

Structure of an uncharacterized protein from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-06
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	74.880, 101.120, 108.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.841 - 1.850
R-factor	0.1708
Rwork	0.169
R-free	0.19970
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc1_3423))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.841	45.841	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.043	0.018	0.559
Rmeas	0.045	0.019	0.613
Number of reflections	35536	445	2591
<I/σ(I)>	37.34	129.03	3.4
Completeness [%]	100.0	98.7	100
Redundancy	10.802	12.211	6.024
CC(1/2)	1.000	1.000	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	290	Molecular Dimensions LMB screen, b10: 24 % (v/v) Ethanol 0.1 M HEPES pH 7.8, 40mM Magnesium chloride: LpboA.19679.a.B1.PS38456 at 15mg/ml, incubation at 4C: cryo: 20% EG: tray 308142 b10: puck kzx0-13. For experimental phasing, a crystal from the same drop was incubated for 20sec in a solution of 80% reservoir and 20% 2.5M Sodium iodide in ethylene glycol, and vitrified: tray: 308142 B10: puck: kzx0-9